Pathogenic variability among Pasteurella multocida type A isolates from Brazilian pig farms

BMC Veterinary Research

Table 3 Target gene information and primers used for Pasteurella multocida type A identification and detection of virulence factors

Gene	Function	Location Gene	N°. Acc.	Primers	DNA-sequences of oligonucleotide primers (5′ – 3′)	Product Size (bp)	Reference
KMT1	Species-specific	213–232	AF016259	KMT1 F	ATCCGCTATTTACCCAGTGG	460	Townsend et al. (2001) [15]
KMT1	Species-specific	669–649	AF016259	KMT1 R	GCTGTAAACGAACTCGCCAC	460
hyaD-hyaC	Capsular synthesis	8846–8863	AF067175	CAPA F	TGCCAAAATCGCAGTCAG	1.044
hyaD-hyaC	Capsular synthesis	9890–9873	AF067175	CAPA R	TTGCCATCATTGTCAGTG	1.044
dcbF	Capsular synthesis	3142–3165	AF302465.	CAPD F	TTACAAAAGAAAGACTAGGAGCCC	657
dcbF	Capsular synthesis	3789–3766	AF302465.	CAPD R	CATCTACCCACTCAACCATATCAG	657
fcbD	Capsular synthesis	2881–2896	AF302467	CAPF F	AATCGGAGAACGCAGAAATCAG	851
fcbD	Capsular synthesis	3733–3714	AF302467	CAPF R	TTCCGCCGTCAATTACTCTG	851
pfhA	Adherence	2409–2427	AY035342	PfhA F	AGCTGATCAAGTGGTGAAC	275	Ewers et al. (2006) [10]
pfhA	Adherence	2684–2665	AY035342	PfhA R	TGGTACATTGGTGAATGGTG	275	Ewers et al. (2006) [10]
tbpA	Iron acquisition	68–85	Pm0337	TbPA F	TTTG GTT GGA AAC GGT AAA GC	728	Ewers et al. (2006) [10] Modified by Atashpaz et al. (2009) [11]
tbpA	Iron acquisition	487–470	Pm0337	TbPA R	TAA CGT GTA CGG AAA AGC CCC	728
hgbB	Iron acquisition	308–328	Pm0337	HgbB F	TCA TTG AGT ACG GCT TGA C	499	Atashpaz et al. (2009) [11]
hgbB	Iron acquisition	1096–1077	Pm0337	HgbB R	CTT ACG TCA GTA ACA CTC G	499	Atashpaz et al. (2009) [11]
toxA	Toxin	1878–1897	AF240778	ToxA F	TTCT TAG ATG AGC GAC AAG G	846	Lichtensteiger et al. (1996) [46] modified by Atashpaz et al. (2009) [11]
toxA	Toxin	2743–2725	AF240778	ToxA R	GAA TGC CAC ACC TCT ATA G	846

ISSN: 1746-6148