Fig. 2

Immunohistochemistry of animals 1 (a-c), 2 (d-f), 3 (g-h) and 4 (i-l) using avidin-biotin-peroxidase and Harris hematoxylin counterstain. a Lung. Intense positive immunoperoxidase staining of morbilliviral antigen (red) within hyperplastic type II pneumocytes and multinucleated syncytial cells. b Prescapular lymph node. Positive intranuclear and intracytoplasmic immunoperoxidase staining of morbilliviral antigen in mononuclear and multinucleated giant and syncytial cells. c Cerebrum. Strong immunolabeling of morbilliviral antigen in neurons, glial cells, and neuronal processes. d Lung. Intense cytoplasmic immunolabeling of alveolar syncytia, pneumocytes and sloughed bronchiolar epithelium. e Lung. Detail of positive cytoplasm immunolabeling of alveolar syncytia and type II pneumocytes. f Cerebrum. Detail of intense immunolabeling of a neuronal body. g Cerebrum. Strong immunolabeling of neurons (× 10). h Cerebrum. Detail of intense immunolabeling of a neuronal body (× 20). i. Lung. (× 10) and j Lung. (× 20). Alveolar spaces containing large numbers of histiocytes, syncytia and sloughed type II pneumocytes with positive cytoplasmic staining against morbillivirus antigen. k Pharyngeal tonsils (× 4) and l Pharyngeal tonsils (× 20). Multifocal histiocytic infiltrates and syncitya with intensely stained cytoplasm against morbillivirus antigen