The molecular characteristic analysis of PRRSV GSWW/2015 strain and its pathogenicity to pigs

BMC Veterinary Research

Table 1 Primers for PCR of the complete genome of PRRSV GSWW/2015 strain

Fragment	Sequence of primers(5′-3′)	Position in genome
A	ATGACGTATAGGTGTTGGCTCTATG	1–1518
A	GGAAATGCAGTGCCAACCACARTTC	1–1518
B	ACTTCTCYGTTAAGGAGAGTTGGAT	1175–4216
B	GATACAGTCTGCAACAATGCCAAGCCTAAGCA	1175–4216
C	GTTACAGTTACCCATTCTTCGG	3911–5191
C	GAGGTACCCAAACATAACTAGC	3911–5191
D	CCTTGGTCGTTTTGATTTTTGT	5081–6304
D	AAGATAGCACAAACATCCCAAAG	5081–6304
E	GGCAGTCACATAATTAAAGACACAT	6061–7292
E	TGGACCTCCTCATAAAACACAT	6061–7292
F	ATTCCGGTGATGTGTTTTATGAGGAGGT	7262–10,030
F	ACATCACCATCCTGGACYTGTTGAAG	7262–10,030
G	TTACCACACCCACTTCCACCAGCATTG	9604–12,066
G	AGAGGCTTTGCATAGACCCCATTTCATTTCAATTCA	9604–12,066
H	TGAATTGAAATGAAATGGGGTCTATGCAAAGCCTCT	11,973–14,584
H	CGGCATCTGGAGGTGATGAATTTCCAGGTTTCTAT	11,973–14,584
I	CCTTCGGGTACATGACATTCGT	14,543–15,347
I	TTTTTTTTTTTTTTTTTTTTTTTTTTTAATTWCGG	14,543–15,347

ISSN: 1746-6148