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Table 1 Immunization records, PCR, culture, DNA sequencing and MAT results found in 33 dogs presenting clinical suspicion of leptospirosis treated at the Veterinary Hospital of University of São Paulo between 2013 and 2016

From: Diagnosis of acute canine leptospirosis using multiple laboratory tests and characterization of the isolated strains

Dog

PCR

DNA sequencing

Culture

Vaccination

MAT

MAT titration (serovar)

 

Blood

Urine

  

(< 1 year)

(> 100)

Can

Po

Cas

Co

Ic

Py

Br

Ha

Wo

Sh

Bu

Aus

Cyn

Aut

1

NP

(+)

L. interrogans

(−)

(+)

(−)

–

–

–

–

–

–

–

–

–

–

–

–

–

–

2

(+)

(+)

L. interrogans

(−)

(−)

(+)

400

200

200

100

200

200

–

–

–

–

–

–

–

–

3

(−)

(+)

L. interrogans

(−)

(−)

(+)

–

800

–

3200

3200

–

200

–

–

–

–

–

–

–

4

(+)

(+)

L. interrogans

(−)

(−)

(+)

–

–

–

–

100

–

–

800

800

–

–

–

–

–

5

NP

(+)

L. interrogans

(−)

(−)

(−)

–

–

–

–

–

–

–

–

–

–

–

–

–

–

6

(−)

(−)

 

(−)

(+)

(+)

–

–

–

–

–

–

–

–

–

200

–

–

–

–

7

NP

(−)

 

(−)

(−)

(+)

–

–

–

–

–

–

–

–

–

–

–

–

100

–

8

(−)

(+)

L. interrogans

(+)

(−)

(+)

–

200

–

400

400

–

–

–

–

–

–

–

–

–

9

(−)

(−)

 

(−)

(+)

(+)

400

–

–

400

200

–

200

–

–

–

100

200

–

–

10

(+)

(−)

L. interrogans

(−)

(−)

(−)

–

–

–

–

–

–

–

–

–

–

–

–

–

–

11

(−)

(−)

 

(−)

(−)

(+)

–

–

–

800

400

–

–

–

–

–

–

–

–

–

12

(−)

(+)

L. interrogans

(−)

(+)

(+)

–

–

–

–

–

–

400

–

–

–

–

–

400

–

13

(−)

(−)

 

(−)

(−)

(−)

–

–

–

–

–

–

–

–

–

–

–

–

–

–

a14

(−)

(−)

 

(−)

(+)

(+)

–

100

–

–

–

–

–

–

–

–

–

–

–

100

a15

(−)

(+)

–

(−)

(−)

(−)

–

–

–

–

–

–

–

–

–

–

–

–

–

–

16

(+)

(−)

L. interrogans

(−)

(−)

(+)

–

–

–

400

200

–

–

–

–

–

200

–

–

–

a17

(+)

(+)

–

(−)

(−)

(−)

–

–

–

–

–

–

–

–

–

–

–

–

–

–

18

(−)

(−)

 

(−)

(+)

(−)

–

–

–

–

–

–

–

–

–

–

–

–

–

–

19

(−)

(−)

 

(−)

(−)

(−)

–

–

–

–

–

–

–

–

–

–

–

–

–

–

20

(−)

(+)

–

(−)

(−)

(+)

–

–

–

400

400

–

–

–

–

–

–

400

–

–

21

(−)

(−)

 

(−)

(−)

(−)

–

–

–

–

–

–

–

–

–

–

–

–

–

–

a22

(−)

(+)

L. interrogans

(−)

(−)

(−)

–

–

–

–

–

–

–

–

–

–

–

–

–

–

23

(−)

(−)

 

(−)

(+)

(+)

–

–

–

200

–

–

–

–

–

–

–

–

–

–

a24

(−)

(+)

L. interrogans

(+)

(+)

(−)

–

–

–

–

–

–

–

–

–

–

–

–

–

–

a25

(−)

(−)

 

(−)

(−)

(−)

–

–

–

–

–

–

–

–

–

–

–

–

–

–

26

(+)

(+)

–

(−)

(+)

(−)

–

–

–

–

–

–

–

–

–

–

–

–

–

–

27

(−)

(−)

 

(−)

(−)

(−)

–

–

–

–

–

–

–

–

–

–

–

–

–

–

28

(−)

(−)

 

(−)

(−)

(−)

–

–

–

–

–

–

–

–

–

–

–

–

–

–

29

(−)

(−)

 

(−)

(−)

(+)

–

–

100

800

400

–

–

–

–

400

–

400

–

–

a30

(−)

(−)

 

(−)

(−)

(+)

–

–

–

200

200

–

–

–

–

–

–

–

–

–

31

(−)

(+)

L. interrogans

(−)

(−)

(+)

–

–

800

–

–

–

–

–

–

–

–

–

–

–

32

(+)

(+)

L. interrogans

(−)

(−)

(+)

–

–

–

1600

–

–

–

–

–

–

200

800

–

–

33

(+)

(+)

L. interrogans

(−)

(−)

(+)

–

–

–

200

100

–

–

–

–

–

100

–

–

–

  1. a Dogs included in the prospective evaluation
  2. NP not performed, Can Canicola, Po Pomona, Cas Castellonis, Co Copenhageni, Ic Icterohaemorrhagiae, Py Pyrogenes, Br Bratislava, Ha Hardjo, Wo Wollfii, Sh Shermani, Bu Butembo, Aus Australis, Cyn Cynopteri, Aut Autumnalis