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Table 1 Immunization records, PCR, culture, DNA sequencing and MAT results found in 33 dogs presenting clinical suspicion of leptospirosis treated at the Veterinary Hospital of University of São Paulo between 2013 and 2016

From: Diagnosis of acute canine leptospirosis using multiple laboratory tests and characterization of the isolated strains

Dog PCR DNA sequencing Culture Vaccination MAT MAT titration (serovar)
  Blood Urine    (< 1 year) (> 100) Can Po Cas Co Ic Py Br Ha Wo Sh Bu Aus Cyn Aut
1 NP (+) L. interrogans (−) (+) (−)
2 (+) (+) L. interrogans (−) (−) (+) 400 200 200 100 200 200
3 (−) (+) L. interrogans (−) (−) (+) 800 3200 3200 200
4 (+) (+) L. interrogans (−) (−) (+) 100 800 800
5 NP (+) L. interrogans (−) (−) (−)
6 (−) (−)   (−) (+) (+) 200
7 NP (−)   (−) (−) (+) 100
8 (−) (+) L. interrogans (+) (−) (+) 200 400 400
9 (−) (−)   (−) (+) (+) 400 400 200 200 100 200
10 (+) (−) L. interrogans (−) (−) (−)
11 (−) (−)   (−) (−) (+) 800 400
12 (−) (+) L. interrogans (−) (+) (+) 400 400
13 (−) (−)   (−) (−) (−)
a14 (−) (−)   (−) (+) (+) 100 100
a15 (−) (+) (−) (−) (−)
16 (+) (−) L. interrogans (−) (−) (+) 400 200 200
a17 (+) (+) (−) (−) (−)
18 (−) (−)   (−) (+) (−)
19 (−) (−)   (−) (−) (−)
20 (−) (+) (−) (−) (+) 400 400 400
21 (−) (−)   (−) (−) (−)
a22 (−) (+) L. interrogans (−) (−) (−)
23 (−) (−)   (−) (+) (+) 200
a24 (−) (+) L. interrogans (+) (+) (−)
a25 (−) (−)   (−) (−) (−)
26 (+) (+) (−) (+) (−)
27 (−) (−)   (−) (−) (−)
28 (−) (−)   (−) (−) (−)
29 (−) (−)   (−) (−) (+) 100 800 400 400 400
a30 (−) (−)   (−) (−) (+) 200 200
31 (−) (+) L. interrogans (−) (−) (+) 800
32 (+) (+) L. interrogans (−) (−) (+) 1600 200 800
33 (+) (+) L. interrogans (−) (−) (+) 200 100 100
  1. a Dogs included in the prospective evaluation
  2. NP not performed, Can Canicola, Po Pomona, Cas Castellonis, Co Copenhageni, Ic Icterohaemorrhagiae, Py Pyrogenes, Br Bratislava, Ha Hardjo, Wo Wollfii, Sh Shermani, Bu Butembo, Aus Australis, Cyn Cynopteri, Aut Autumnalis