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Table 1 Immunoreactivity of normal tissues from two reptile species [bearded dragon (Pogona vitticeps) and loggerhead sea turtle (Caretta caretta)] with commercially available monoclonal anti-alpha human keratin AE1 and AE3 antibodies

From: Immunohistochemical staining patterns of alpha-keratins in normal tissues from two reptile species: implications for characterization of squamous cell carcinomas

 

Pogona vitticeps

Caretta caretta

AE1

AE3

AE1

AE3

Epidermis (n = 3/4)a

 Basal cells

+

+++

++

 Suprabasal cells

+

+++

+++

 Pre-cornified layers

+++/++b

+

++

 Cornified layer

+++c

Lingual epithelium (n = 0/4)

ND

ND

+++d

+d

Oesophageal epithelium (n = 0/6)

ND

ND

+++d

+++e

Gastric mucosa (n = 4/6)

 Surface epithelium

++

+++/−f

+

 Gastric glands

+/−

Small intestine epithelium (n = 4/6)

+++

+++

Large intestine epithelium (n = 4/6)

Liver (n = 4/6)

 Hepatocytes

++

++

 Biliary ductal epithelium

+++

+++

Gallbladder epithelium (n = 0/3)

ND

ND

+

Pancreas (n = 2/5)

 Acinar epithelium

 Ductal epithelium

Tracheal epithelium (n = 4/6)

++

+++

Lung (bronchial, bronchiolar and faveolar epithelia) (n = 4/6)

++

+++

Kidney (n = 4/6)

 Parietal layer of Bowman’s capsule

+++

+/−

 Proximal tubules

 Distal tubules

++

+++

+++

 Collecting ducts

+

+++

+++

Urinary bladder epithelium (n = 0/3)

NA

NA

+/−

Salt gland (n = 0/6)

NA

NA

  

 Secretory acini

  

+++/+g

+/−

 Ductal epithelium

  

+++

+++

Thyroid gland epithelium (n = 2/5)

Spleen (n = 4/6)

Epicardium (n = 4/6)

+

+

Vascular endothelium (n = 4/6)

+/−

  1. Reactivity was judged as intense (+++), moderate (++), weak (+), negative (−), or variable (weak/negative) (+/−), respectively
  2. ND not done due to lack of samples
  3. NA not applicable (the species does not have this organ)
  4. aNumber of cases for each individual organ or tissue (n = x/y), where ‘x’ stands for the number of tissues from bearded dragons and ‘y’ for that of loggerhead sea turtles
  5. bReactivity within the cytoplasm of suprabasal cells incorporated into the alpha-layer
  6. cReactivity limited to the inner cornified layer (alpha-layer)
  7. dReactivity limited to the basal and suprabasal cells of the epithelium
  8. eReactivity in all layers of the epithelium (except in the cornified layer) but more intense in the suprabasal cells
  9. fReactivity was detected in the majority of the epithelial cells, but some cells were negative
  10. gIntense reactivity in the peripheral secretory acini and weak reactivity in the others