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Fig. 5 | BMC Veterinary Research

Fig. 5

From: Immunohistochemical staining patterns of alpha-keratins in normal tissues from two reptile species: implications for characterization of squamous cell carcinomas

Fig. 5

Metastases of squamous cell carcinoma, heart, loggerhead turtle (Caretta caretta) No. 2. a Immunohistochemistry for acidic alpha-keratin (AE1 positive) showing intense immunoreactivity in all cells of the metastatic foci. Bar = 80 μm. b Immunohistochemistry for basic alpha-keratin (AE3 positive) showing intense immunoreactivity only in the suprabasal and pre-cornified layers of a big metastatic focus. Bar = 110 μm. Dako EnVision+ System, Harris haematoxylin counterstain

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