Table 2 Primer sequences for real-time PCR analysis
Gene | Sequence(5′ → 3′) | Product size (bp) | GenBank accession number |
|---|---|---|---|
ZO-1 | CTCTTGGCTTGCTATTCG | 256 | XM_003353439.2 |
AGTCTTCCCTGCTCTTGC | |||
Occludin | GTAGTCGGGTTCGTTTCC | 167 | NM_001163647.2 |
GACCTGATTGCCTAGAGTGT | |||
IL-1β | CTCCAGCCAGTCTTCATTGTTC | 132 | NM_214055.1 |
TGCCTGATGCTCTTGTTCCA | |||
TNF-α | CACCACGCTCTTCTGCCTAC | 116 | X54859 |
ACGGGCTTATCTGAGGTTTGAGACG | |||
IL-10 | GGTTGCCAAGCCTTGTCAG | 202 | NM_214041 |
AGGCACTCTTCACCTCCTC | |||
TGF-β | GAAGCGCATCGAGGCCATTC | 162 | NM_214015 |
GGCTCCGGTTCGACACTTTC | |||
β-actin | CGGGACATCAAGGAGAAGC | 273 | DQ845171 |
ACAGCACCGTGTTGGCGTAGAG |