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Table 4 Performance of combinations of bead sets in comparison with a commercial BC1D1 ELISA

From: A bead-based suspension array for the detection of Salmonella antibodies in pig sera

   B beads   
   pos neg total Relative sensitivity 91%
ELISA (BC1D1) pos 58 6 64 Relative specificity 98%
neg 2 109 111 Efficiency 95%
total 60 115 175 Cohen’s kappa 0.90
   B + D1 beads   
   pos neg total Relative sensitivity 94%
ELISA (BC1D1) pos 60 4 64 Relative specificity 98%
neg 2 109 111 Efficiency 97%
total 62 113 175 Cohen’s kappa 0.93
   B + D1 + C1 + C1 beads   
   pos neg total
ELISA (BC1D1) pos 61 3 64
neg 13 98 111
  total 74 101 175
   B + D1 + C1 + C1 + C2 beads
   pos neg total
ELISA (BC1D1) pos 61 3 64
neg 17 94 111
total 78 97 175
  1. For four different combinations of LPS-conjugated bead sets, the results on individual bead sets were used to assign a serostatus to each of the 175 tested sera from slaughter pigs. Sera were designated seropositive when a signal above the threshold was detected with at least one of the bead sets. The resulting designations were used to determine the performance of the various bead set combinations. Only for B and D beads (see Methods) relative sensitivity, relative specificity, efficiency, and Cohen’s kappa were determined [26], using the BC1D1 ELISA as criterion