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Table 4 Performance of combinations of bead sets in comparison with a commercial BC1D1 ELISA

From: A bead-based suspension array for the detection of Salmonella antibodies in pig sera

  

B beads

  
  

pos

neg

total

Relative sensitivity

91%

ELISA (BC1D1)

pos

58

6

64

Relative specificity

98%

neg

2

109

111

Efficiency

95%

total

60

115

175

Cohen’s kappa

0.90

  

B + D1 beads

  
  

pos

neg

total

Relative sensitivity

94%

ELISA (BC1D1)

pos

60

4

64

Relative specificity

98%

neg

2

109

111

Efficiency

97%

total

62

113

175

Cohen’s kappa

0.93

  

B + D1 + C1 + C1 beads

  
  

pos

neg

total

ELISA (BC1D1)

pos

61

3

64

neg

13

98

111

 

total

74

101

175

  

B + D1 + C1 + C1 + C2 beads

  

pos

neg

total

ELISA (BC1D1)

pos

61

3

64

neg

17

94

111

total

78

97

175

  1. For four different combinations of LPS-conjugated bead sets, the results on individual bead sets were used to assign a serostatus to each of the 175 tested sera from slaughter pigs. Sera were designated seropositive when a signal above the threshold was detected with at least one of the bead sets. The resulting designations were used to determine the performance of the various bead set combinations. Only for B and D beads (see Methods) relative sensitivity, relative specificity, efficiency, and Cohen’s kappa were determined [26], using the BC1D1 ELISA as criterion