Development and application of a monoclonal antibody-based blocking ELISA for detection of antibodies to Tembusu virus in multiple poultry species

Table 1 Tembusu virus (TMUV) seroprevalence in domestic ducks in Northern China, 2009–2015

Sampling date	Sampling site	Species	No. sera	b-ELISA positive	Positive rate
2009	Hebei	laying duck	120	0	0%
2009	Beijing	laying duck	80	0	0%
2011.03	Hebei	layingduck^a	289	289	100%
2011.03	Beijing	breeding duck^a	80	80	100%
2011.03	Shandong	breeding duck^a	60	60	100%
2012. 11	Beijing	laying duck^b	152	18	11.84%
2012. 12	Beijing	laying duck^b	96	9	9.38%
2013. 01	Beijing	laying duck^b	122	12	9.83%
2013.03	Beijing	table duckling^b	90	0	0%
2013. 04	Beijing	table duckling^b	176	0	0%
2013.06	Beijing	laying duck^b	180	6	3.33%
2013.08	Beijing	laying duck^b	190	25	13.16%
2013.12	Beijing	table duckling^b	106	4	3.77%
2015.05	Beijing	table duckling^b	50	0	0%
2015.09	Hebei	breeding duck^b	80	0	0%
2015.12	Hebei	breeding duck^c	241	79	32.78%
2015.12	Beijing	breeding duck^c	237	226	95.36%

^aThe sera were from the animals suffered from DTMUV infection in December 2010
^bThe sera were from the animals with no history of DTMUV infection and immunization
^cThe sera were from the animals immunized with autogenous vaccine of DTMUV within one year

ISSN: 1746-6148