Fig. 1From: Development and application of a monoclonal antibody-based blocking ELISA for detection of antibodies to Tembusu virus in multiple poultry speciesCharacterization of the MAb 9E4 against TMUV. a Indirect immunofluorescence assay and immunocytochemistry of MAb 9E4 against duck TMUV, Japanese encephalitis virus and batai virus-infected BHK-21 cells. b Western blot analysis of MAb 9E4 reactivity against the virus particle. 1&2: Virus particle under non-reducing conditions or reducing conditions; 3&4: BHK-21 cell lysates under non-reducing conditions or reducing conditions; c Western blot analysis of MAb 9E4 reactivity against disulfide bonds reformed recombinant expression protein under non-reducing condition. 1: pET 32Ī± tag protein; 2: E ectodomain; 3: E domain I/II; 4: E domain IIIBack to article page