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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Development and application of a monoclonal antibody-based blocking ELISA for detection of antibodies to Tembusu virus in multiple poultry species

Fig. 1

Characterization of the MAb 9E4 against TMUV. a Indirect immunofluorescence assay and immunocytochemistry of MAb 9E4 against duck TMUV, Japanese encephalitis virus and batai virus-infected BHK-21 cells. b Western blot analysis of MAb 9E4 reactivity against the virus particle. 1&2: Virus particle under non-reducing conditions or reducing conditions; 3&4: BHK-21 cell lysates under non-reducing conditions or reducing conditions; c Western blot analysis of MAb 9E4 reactivity against disulfide bonds reformed recombinant expression protein under non-reducing condition. 1: pET 32α tag protein; 2: E ectodomain; 3: E domain I/II; 4: E domain III

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