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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Safety and immunogenicity of an attenuated Chinese pseudorabies variant by dual deletion of TK&gE genes

Fig. 2

Restriction fragment length polymorphism (RFLP) of BACPRV-G and its TK deletion mutants. a RFLP pattern of BAC PRVTK/gE/gI, BACPRVTK/gE/gI&K+ and BACPRV-G. Lane 1, 2 and 3 are BAC PRVTK/gE/gI, BACPRVTK/gE/gI&K+ and BACPRV-G respectively after digestion with Kpn I. The arrow (lane 2) shows an additional band of 6639 bp and a 5975 bp band missed when compared to lane 3. The arrow(lane 1) showed an additional band of 5628 bp and a missing band of 6639 bp when compared to lane 2. M is 1Kb DNA marker. b Predicted RFLP pattern with PRV ZJ01 strain (GenBank:KM061380.1) as a reference. Lane 1, 2 and 3 are prediction of BAC PRVTK/gE/gI, BACPRVTK/gE/gI&K+ and BACPRV-G respectively digested with Kpn I. c Kpn I sites were cited in lane 1, 2 and 3 for BAC PRVTK/gE/gI, BACPRVTK/gE/gI&K+ and BACPRV-G respectively. Sites underlined with red lines indicate the changed position of Kpn I restriction site leading to the bands changing accordingly

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