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Table 2 Summary of cytology, bacteriology results and NET quantification

From: A novel approach to identifying and quantifying neutrophil extracellular trap formation in septic dogs using immunofluorescence microscopy

  Differential cytology Cytological diagnosis Bacteriology NET (NET:cells) Ratio of intracellular citH3
Endotracheal Tracheal Lavage  
 Dog 1 TNCC: 10.36 × 103/μL
Neutrophils: 85%
Lymphocytes: 0%
Macrophages: 15%
Eosinophils: 0%
Marked suppurative inflammation Staphylococcus intermedius, Corynebacterium sp., Mycoplasma sp. 0.070 0.093
Pleural effusion
 Dog 2 TNCC: 482 × 103 /μL
Neutrophils: 78%
Macrophages: 20%
Others: 2%
Marked septic suppurative inflammation Streptococcus Viridans
Fusobacterium nucleatum
0.26 0.67
Abdominal Fluid  
 Dog 3 TNCC: 21.6 × 103/μL
Neutrophils: 84%
Lymphocytes:12%
Macrophages: 4%
Eosinophils: 0%
Moderate septic suppurative inflammation Enterococcus faecalis
Enterococcus faecium
0.14 0.24
Bronchoalveolar Lavage
 Dog 4 TNCC: 0.54 × 103/μL
Neutrophils: 17%
Lymphocytes: 18%
Macrophages: 41%
Eosinophils: 24%
Mild mixed inflammation Negative 0.016 0.032
Pleural Effusion  
 Dog 5 TNCC: 2.5 × 103/μL
Neutrophils: 28%
Lymphocytes: 1%
Macrophages: 69%
Others: 2%
Mild mixed inflammation Negative 0 0.0032
  1. TNCC total nucleated cell count
  2. NET neutrophil extracellular trap
  3. CitH3 citrullinated histone H3