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Table 2 Summary of cytology, bacteriology results and NET quantification

From: A novel approach to identifying and quantifying neutrophil extracellular trap formation in septic dogs using immunofluorescence microscopy

 

Differential cytology

Cytological diagnosis

Bacteriology

NET (NET:cells)

Ratio of intracellular citH3

Endotracheal Tracheal Lavage

 

 Dog 1

TNCC: 10.36 × 103/μL

Neutrophils: 85%

Lymphocytes: 0%

Macrophages: 15%

Eosinophils: 0%

Marked suppurative inflammation

Staphylococcus intermedius, Corynebacterium sp., Mycoplasma sp.

0.070

0.093

Pleural effusion

 Dog 2

TNCC: 482 × 103 /μL

Neutrophils: 78%

Macrophages: 20%

Others: 2%

Marked septic suppurative inflammation

Streptococcus Viridans

Fusobacterium nucleatum

0.26

0.67

Abdominal Fluid

 

 Dog 3

TNCC: 21.6 × 103/μL

Neutrophils: 84%

Lymphocytes:12%

Macrophages: 4%

Eosinophils: 0%

Moderate septic suppurative inflammation

Enterococcus faecalis

Enterococcus faecium

0.14

0.24

Bronchoalveolar Lavage

 Dog 4

TNCC: 0.54 × 103/μL

Neutrophils: 17%

Lymphocytes: 18%

Macrophages: 41%

Eosinophils: 24%

Mild mixed inflammation

Negative

0.016

0.032

Pleural Effusion

 

 Dog 5

TNCC: 2.5 × 103/μL

Neutrophils: 28%

Lymphocytes: 1%

Macrophages: 69%

Others: 2%

Mild mixed inflammation

Negative

0

0.0032

  1. TNCC total nucleated cell count
  2. NET neutrophil extracellular trap
  3. CitH3 citrullinated histone H3