Fig. 3

T-cell proliferation assay of PBMC from goats immunized with BVDV vaccines. Goats were immunized twice intramuscularly with four different vaccine formulations: 1) iBVDV+rE2, 2) rE2, 3) iBVDV, and 4) saline. PBMC from blood were isolated and stimulated with inactivated BVDV or Con A (as a positive control). Proliferation extent was measured and stimulation index (SI) calculated. Data represent means ± SD. Differences between groups were analyzed by Duncan’s significance test and different letters indicate significant difference at p < 0.05