Fig. 9

Antiviral activity analysis of Lfcin expressed by ΔthyA L. casei-LFEC. Positive, the inhibition of PEDV replication with the addition of native bovine lactoferricin; Negative control, the inhibition of PEDV replication with the addition of the supernatant of cultured L. casei; Lfcin, the inhibition of PEDV replication with the addition of the supernatant of cultured ΔthyA L. casei-LFEC; (a) The supernatant was incubated with VERO cells before PEDV adsorption; (b) The supernatant were simultaneously added to VERO cells with PEDV; (c) The supernatant were added to VERO cells after PEDV adsorption