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Table 3 Results of M. hyopneumoniae-DNA detection in the BALF and serology in the different experimental groups

From: Clinical impact of deoxynivalenol, 3-acetyl-deoxynivalenol and 15-acetyl-deoxynivalenol on the severity of an experimental Mycoplasma hyopneumoniae infection in pigs

Parameter Groups
  NCG (n = 5) DON (n = 15) DONMHYO (n = 15) MHYO (n = 15) P-value
qPCR (log copies of M. hyopneumoniae DNA /ml BALF) ± SD
 D21 0.05 ± 0.37a 0.63 ± 0.81a 3.92 ± 2.60b 3.24 ± 1.91b < 0.001
 D35/36 0.80 ± 0.70a 0.29 ± 1.09a 4.05 ± 1.38b 4.20 ± 1.00b < 0.001
Percentage of M. hyopneumoniae qPCR positive samples (n of positive samples/total n of samples tested)
 D21 (%) 0 (0/5)a 0 (0/15)a 87 (13/15)b 93 (14/15)b < 0.001
 D35/36 (%) 0 (0/5)a 0 (0/15)a 100 (15/15)b 100 (15/15)b < 0.001
M. hyopneumoniae specific AB expressed in OD-values ± SD
 D8 1.68 ± 0.23 1.83 ± 0.34 1.71 ± 0.19 1.70 ± 0.19 0.27
 D21 1.15 ± 0.22a 1.29 ± 0.23a 1.16 ± 0.36ab 0.94 ± 0.26b 0.001
 D35/36 1.15 ± 0.27a 1.16 ± 0.18a 0.25 ± 0.18b 0.23 ± 0.10b < 0.001
Percentage of ELISA M. hyopneumoniae positive samples (n of positive samples/total n of samples tested)
 D8 (%) 0 (0/5) 0 (0/15) 0 (0/15) 0 (0/15) 1.00
 D21 (%) 0 (0/5) 0 (0/15) 0 (0/15) 13 (2/15) 0.17
 D35/36 (%) 0 (0/5)a 0 (0/15)a 100 (15/15)b 100 (15/15)b < 0.001
  1. Respiratory disease score (RDS), bodyweight, average daily weight gain (ADG), macroscopic lung lesions (MLL), histopathology score of the lungs and percentage of air in the lungs for NCG, DON group, DONMHYO group, MHYO group
  2. The parameter qPCR and M. hyopneumoniae specific AB were analysed with a non-parametric Kruskal-Wallis and the Dunn-Bonferroni approach to make pair-wised comparisons, therefore median and interquartile range are reported. The prevalence of M. hyopneumoniae qPCR positive samples and the prevalence of ELISA M. hyopneumoniae positive samples were analysed with binomial logistic regression. Different superscripts in one row are statistically different (P < 0.05)
  3. NCG: negative control group, DON: deoxynivalenol contaminated group, DONMHYO: deoxynivalenol contaminated + M. hyopneumoniae-inoculated group, MHYO: M. hyopneumoniae-inoculated group, Different superscripts in one row are statistically different (P < 0.05), n number, SD standard deviation, D Day of the study, M. hyopneumoniae: Mycoplasma hyopneumoniae, AB antibodies, OD optical densities