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Table 1 Experimental design, sample collections and timing in the different experimental groups

From: Clinical impact of deoxynivalenol, 3-acetyl-deoxynivalenol and 15-acetyl-deoxynivalenol on the severity of an experimental Mycoplasma hyopneumoniae infection in pigs

Study day, D Groups
NCG (n = 5) DON (n = 15) DONMHYO (n = 15) MHYO (n = 15)
D0a Arrival
D0-D35/36b Commercial feed ad libitum DON-contaminated feed ad libitum DON-contaminated feed ad libitum Commercial feed ad libitum
D1-D35c RDS
D8 Sham-inoculationd Sham-inoculationd F7.2Ce-inoculation F7.2Ce-inoculation
D9 Sham-inoculationd Sham-inoculationd F1.12Af-inoculation F1.12Af-inoculation
D35/36b Weight
Lung sample
  1. athe average age of the pigs at arrival was 26 days, ball pigs of the NCG and DON group, and five pigs of MHYO were necropsied at D35. Ten pigs of MHYO and all pigs of DONMHYO were necropsied at D36, cRDS was not determined at D0 and D36 because the piglets arrived later than 8 a.m. (hour of performing coughing score every day) and part of the piglets were already euthanized on D36, respectively, dsham-inoculation was performed with sterile Friis medium, ehighly virulent strain of M. hyopneumoniae, flow virulent strain of M. hyopneumoniae
  2. NCG negative control group, DON deoxynivalenol contaminated group, DONMHYO deoxynivalenol contaminated +M. hyopneumoniae-inoculated group, MHYO M. hyopneumoniae-inoculated group, RDS respiratory disease score, BALF bronchoalveolar lavage fluid