Skip to main content

Table 2 Detection limits of one-step RT-PCR and RtF-RT-LAMP for the NK94P6 strain

From: Development of pooled testing system for porcine epidemic diarrhoea using real-time fluorescent reverse-transcription loop-mediated isothermal amplification assay

TCID50

2.8 × 105

2.8 × 104

2.8 × 103

2.8 × 102

2.8 × 101

2.8 × 100

2.8 × 10− 1

One-step

RT-PCR

+

+

+

–

–

–

 

RtF-RT-LAMP

(amplification time mm:ss)

+

(9:30)

+

(11:45)

+

(12:30)

+

(15:45)

+

(34:45)

–

–

  1. + Positive in duplicate
  2. - Negative in duplicate
  3. From 2.8 × 105 to 2.8 × 10− 1: tenfold serial dilution of 2.8 × 106 TCID50 PEDV NK94P6 strain
Back to article page

BMC Veterinary Research

ISSN: 1746-6148

Contact us