Table 3 Oliconucleotide primers used for RT-qPCR
From: Inflammatory pattern of the infrapatellar fat pad in dogs with canine cruciate ligament disease
Target gene | Forward (F) and reverse (R) primer (5′-3′) | Amplicon size (bp) | Annealing temperature (°C) |
|---|---|---|---|
IL-1β | F-GCCAAGACCTGAACCACAGT | 96 | 60.16 |
R-CTGACACGAAATGCCTCAGA | 59.98 | ||
IL-6 | F-ACCGGTCTTGTGGAGTTTCA | 102 | 60.55 |
R-CAGGATCTTGGTACTCATGTGC | 59.62 | ||
IL-8 | F-TTCAGAACTTCGATGCCAGT | 90 | 58.44 |
R-GGGCCACTGTCAATCACTCT | 60.12 | ||
IL-10 | F-CAGAGCACCCTACCTGAGGA | 97 | 60.40 |
R-AAGTCTTCACCCTCCCGAAG | 60.62 | ||
TNFα | F-GAGCACTGAAAGCATGATCC | 108 | 58.39 |
R-GAGAAGAGGCTGAGGCAGAA | 59.83 | ||
iNOS | F-GTCTGGGAGCCATCATGAAC | 98 | 60.48 |
R-GACAAATTCAATGGCTTGAGG | 59.56 | ||
MMP-1 | F-TGAATTGGGTCATTCTCTTGG | 98 | 59.92 |
R-CCTGAGATAGCTGGACATTGC | 59.85 | ||
MMP-3 | F-CCTAGCGCTCTGATGTACCC | 90 | 59.86 |
R-GGACTGGATGCCATTCACAT | 60.76 | ||
MMP-13 | F-AGTTCGGCCACTCCTTAGGT | 102 | 60.13 |
R-CATCGGGAAGCATAAAGTGG | 60.46 | ||
TIMP-2 | F-TGTTCAAAGGACCAGACAAGG | 109 | 60.13 |
R-TTCTTTCCTCCGATGTCCAG | 60.19 | ||
β-actin | F-GGACCTGACCGACTACCTCA | 91 | 60.11 |
R-CTTGATGTCACGCACGATTT | 59.72 |