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Table 3 Oliconucleotide primers used for RT-qPCR

From: Inflammatory pattern of the infrapatellar fat pad in dogs with canine cruciate ligament disease

Target gene Forward (F) and reverse (R) primer (5′-3′) Amplicon size (bp) Annealing temperature (°C)
IL-1β F-GCCAAGACCTGAACCACAGT 96 60.16
R-CTGACACGAAATGCCTCAGA 59.98
IL-6 F-ACCGGTCTTGTGGAGTTTCA 102 60.55
R-CAGGATCTTGGTACTCATGTGC 59.62
IL-8 F-TTCAGAACTTCGATGCCAGT 90 58.44
R-GGGCCACTGTCAATCACTCT 60.12
IL-10 F-CAGAGCACCCTACCTGAGGA 97 60.40
R-AAGTCTTCACCCTCCCGAAG 60.62
TNFα F-GAGCACTGAAAGCATGATCC 108 58.39
R-GAGAAGAGGCTGAGGCAGAA 59.83
iNOS F-GTCTGGGAGCCATCATGAAC 98 60.48
R-GACAAATTCAATGGCTTGAGG 59.56
MMP-1 F-TGAATTGGGTCATTCTCTTGG 98 59.92
R-CCTGAGATAGCTGGACATTGC 59.85
MMP-3 F-CCTAGCGCTCTGATGTACCC 90 59.86
R-GGACTGGATGCCATTCACAT 60.76
MMP-13 F-AGTTCGGCCACTCCTTAGGT 102 60.13
R-CATCGGGAAGCATAAAGTGG 60.46
TIMP-2 F-TGTTCAAAGGACCAGACAAGG 109 60.13
R-TTCTTTCCTCCGATGTCCAG 60.19
β-actin F-GGACCTGACCGACTACCTCA 91 60.11
R-CTTGATGTCACGCACGATTT 59.72