Fig. 3
From: Inflammatory pattern of the infrapatellar fat pad in dogs with canine cruciate ligament disease
Gene expression and protein release of ScAT and IFP and cytokine concentration in synovial fluid. mRNA was quantified by real time RT PCR (RT qPCR). Values for MMP-1, MMP-3, MMP-13 and TIMP-2 were normalized to the housekeeping gene (β-actin) and presented as the fold increase compared to control ScAT on the left y-axis. Protein concentration of MMP-1, MMP-3 and MMP-13 secreted by ScAT and IFP as well as in synovial fluid were measured by ELISA and presented as ng/ ml on the right y-axis. * denotes statistically significant differences with p < 0.05 and ** p < 0.01. White boxes demonstrate control group and grey boxes samples from dogs with CCLD