Skip to main content

Table 1 Chemical characterization of the aqueous extract of Maytenus senegalensis stems bark

From: In vitro effects of aqueous extract from Maytenus senegalensis (Lam.) Exell stem bark on egg hatching, larval migration and adult worms of Haemonchus contortus

No.

t R

(min)

[M-H]−

m/z

m/z (% base peak)

Assigned identification

1

1.2

191

MS2 [191]: 173 (31), 129 (3), 111 (100)

Citric acid

2

1.6

879

MS2 [879]: 879 (100), 861 (79), 727 (61), 709 (47), 529 (16), 287 (38)

MS3 [879 → 861]: 843 (100), 709 (49), 573 (84), 411 (75), 285 (48)

(Epi)gallocatechin-(epi)catechin-(epi)catechin

3

2.3

593

MS2 [593]: 575 (29), 467 (31), 441 (63), 423 (100), 305 (64), 289 (7), 287 (7)

(Epi)catechin-(epi)gallocatechin (B-type)

4

2.3

577

MS2 [577]: 575 (16), 451 (13), 425 (100), 407 (72), 289 (28), 287 (14), 245 (6)

(Epi)catechin-(epi)catechin (B-type)

5

2.6

865

MS2 [865]: 739 (87), 713 (54), 695 (100), 577 (80), 575 (51), 289 (6), 287 (27)

(Epi)catechin-(epi)catechin-(epi)catechin (B-type)

6

3.1

577

MS2 [577]: 575 (6), 451 (14), 425 (100), 407 (74), 289 (22), 287 (41)

(Epi)catechin-(epi)catechin (B-type)

7

3.1

289

MS2 [289]: 245 (100), 205 (23), 203 (23), 125 (13)

Catechin

8

3.4

595

MS2 [595]: 577 (9), 505 (12) 475 (44), 415 (16), 385 (89), 355 (100), 313 (7)

Naringenin-6,8-di-C-hexoside

9

3.6

577

MS2 [577]: 575 (4), 451 (12), 425 (100), 407 (57), 289 (16), 287 (11)

(Epi)catechin-(epi)catechin (B-type)

10

4.3

581

MS2 [581]: 419 (100)

MS3 [581 → 419]: 373 (14), 404 (100)

MS4 [581 → 419 → 404]: 389 (100), 373 (38)

Methylated flavonoid-O-hexoside

11

4.7

289

MS2 [289]: 245 (100), 205 (37), 203 (18), 179 (21), 125 (9)

Epicatechin

12

5.8

865

MS2 [865]: 739 (53), 713 (35), 695 (100), 577 (68), 575 (30), 289 (6), 287 (18)

(Epi)catechin-(epi)catechin-(epi)catechin (B-type)

13

6.4

755

MS2 [755]: 755 (100), 301 (7), 300 (10)

MS3 [755 → 300]: 271 (100), 255 (70), 151 (89)

Quercetin derivative

14

6.5

741

MS2 [741]: 741 (100), 301 (12)

MS3 [741 → 301]: 255 (100), 151 (56)

Quercetin derivative

15

6.6

577

MS2 [577]: 575 (15), 451 (21), 425 (100), 407 (72), 289 (41), 287 (13), 245 (12)

(Epi)catechin-(epi)catechin (B-type)

16

7.5

739

MS2 [739]: 739 (100), 285 (11), 284 (9)

MS3 [739 → 284]: 255 (100), 241 (17), 151 (28)

Kaempferol derivative

17

7.9

593

MS2 [593]: 447 (100), 301 (49)

MS3 [593 → 447]: 301 (100), 300 (65), 271 (50), 255 (31), 179 (42)

Quercetin-O-di-rhamnoside

18

8.0

609

MS2 [609]: 301 (100)

MS3 [609 → 301]: 271 (15), 255 (12), 179 (54), 151 (100)

Rutin

19

8.4

463

MS2 [463]: 317 (70), 316 (100)

MS3 [463 → 316]: 271 (100), 179 (48), 151 (14)

Myricetin-O-rhamnoside

20

8.5

593

MS2 [593]: 285 (80), 284 (100)

MS3 [593 → 284]: 255 (100), 151 (23)

Kaempferol-O-rutinoside

21

8.8

309

MS2 [309]: 193 (100)

MS3 [309 → 193]: 178 (100), 149 (50), 134 (85)

Ferulic acid derivative

22

9.0

597

MS2 [597]: 477 (64), 459 (15), 417 (13), 387 (45), 357 (100), 315 (11)

Phloretin-di-C-hexoside

23

9.7

433

MS2 [433]: 301 (100), 300 (77)

MS3 [433 → 301]: 271 (100), 255 (94), 151 (54)

Quercetin-O-pentoside

24

9.8

505

MS2 [505]: 301 (100), 300 (40)

MS3 [505 → 301]: 271 (96), 255 (18), 179 (100), 151 (87)

Quercetin-O-acetylhexoside

25

9.9

593

MS2 [593]: 447 (100)

MS3 [593 → 447]: 301 (100), 300 (21)

MS4 [593 → 447 → 301]: 179 (100), 151 (62)

Quercetin-O-di-rhamnoside

26

10.4

563

MS2 [563]: 417 (13), 285 (90), 284 (100)

MS3 [563 → 284]: 257 (74), 255 (100), 227 (84), 151 (41)

Kaempferol-O-rhamnosylpentoside

27

10.5

447

MS2 [447]: 301 (100)

MS3 [447 → 301]: 255 (100), 179 (18)

Quercetin-O-rhamnoside

28

10.8

577

MS2 [577]: 577 (100), 431 (16), 285 (11)

MS3 [577 → 431]: 284 (90), 285 (100)

MS4 [577 → 431 → 284]: 255 (40), 151 (100)

Kaempferol-O-di-rhamnoside

29

11.0

489

MS2 [489]: 285 (66), 284 (100)

MS3 [489 → 284]: 255 (100), 227 (29)

Kaempferol-O-acetylhexoside

30

12.1

431

MS2 [431]: 284 (35), 285 (100), 255 (11), 227 (5)

Kaempferol-O-rhamnoside

31

12.8

329

MS2 [329]: 314 (100)

MS3 [329 → 314]: 299 (100), 269 (71)

Dimethylated flavonoid

32

13.2

357

MS2 [357]:168 (7), 167 (100)

MS3 [357 → 167]: 123 (100)

Vanillic acid derivative

33

13.6

483

MS2 [483]: 437 (100)

MS3 [483 → 437]: 291 (100)

Flavonoid rhamnoside (formate adduct)

34

19.9

327

MS2 [327]: 291 (12), 229 (12), 211 (5), 171 (100)

Oxo-dihydroxy-octadecenoic acid

35

20.5

327

MS2 [327]: 291 (24), 229 (79), 211 (47), 209 (10), 171 (100)

Oxo-dihydroxy-octadecenoic acid

36

25.6

329

MS2 [329]: 311 (21), 293 (28), 229 (100), 211 (62), 171 (82)

Trihydroxy-octadecenoic acid

37

26.2

397

MS2 [397]: 351 (100), 329 (77), 193 (20)

MS3 [397 → 351]: 251 (87), 233 (78), 193 (100)

Unknown (formate adduct)

  1. The initial step for the characterization of the phenolic compounds consisted in the determination of the molecular weight of each compound. The qualitative analysis of the aqueous extract of M. senegalensis by HPLC-ESI-MSn obtained correspond to the ESI− mode, whereas the ESI+ mode was used to confirm the proanthocyanidins structures. Approximately 70 % of the compounds detected in the extracts were proanthocyanidins (20%) and flavonoids (> 50%). Esquire control software was used for the data acquisition and data analysis for processing
Back to article page

BMC Veterinary Research

ISSN: 1746-6148

Contact us