Skip to main content

Table 6 Blood immune cell percentages, cecal luminal sIgA contents and the mRNA expression of cytokines in cecal tonsils after indicated treatments1

From: Responses to dietary levels of methionine in broilers medicated or vaccinated against coccidia under Eimeria tenella-challenged condition

  Medicated groups Vaccinated groups P value
Met2 concentrations: 0.45% 0.56% 0.68% 0.45% 0.56% 0.68% Anticoccidial program Met levels Anticoccidial program × Met levels
CD4 (%) 9.72 ± 1.05c 12.27 ± 1.36a 12.12 ± 1.33a 12.95 ± 1.42a 10.85 ± 1.27b 10.61 ± 1.17bc 0.092 0.028 0.002
CD8 (%) 8.51 ± 0.92d 11.16 ± 1.23a 10.96 ± 1.08ab 10.24 ± 1.14b 9.51 ± 1.03bc 9.21 ± 0.99c 0.045 0.015 0.005
CD4/CD8 1.15 ± 0.11b 1.10 ± 0.11b 1.11 ± 0.13b 1.27 ± 0.12a 1.14 ± 0.12b 1.16 ± 0.13b 0.023 0.041 0.003
sIgA (mg/mL) 1.30 ± 0.19b 1.47 ± 0.22a 1.52 ± 0.27a 1.50 ± 0.24a 1.49 ± 0.21a 1.50 ± 0.28a 0.028 0.037 0.008
TNF-α 1.09 ± 0.11b 1.39 ± 0.20a 1.47 ± 0.26a 0.83 ± 0.09c 0.87 ± 0.10c 0.81 ± 0.07c 0.008 0.035 0.025
IL-2 1.04 ± 0.09b 1.27 ± 0.14a 1.23 ± 0.18a 0.97 ± 0.11b 0.95 ± 0.09b 0.98 ± 0.07b 0.013 0.039 0.031
IFN-γ 1.10 ± 0.14e 1.54 ± 0.25c 1.62 ± 0.29b 1.76 ± 0.33a 1.33 ± 0.22d 1.14 ± 0.13e 0.074 0.005 0.001
  1. 1An anticoccidial drug (narasin, 60 mg/kg diet) was added to diets in medicated groups, and an anticoccidial 7-valent live vaccine was inoculated in vaccinated groups
  2. 2Met methionine, CD4 CD4+ cells, CD8 CD8+ cells, sIgA secreted immunoglobulin A, TNF-α tumor necrosis factor-α, IL-2 interleukin-2, IFN-γ interferon-γ
  3. a,b,c,d,eThe data of the same row not sharing a lowercase letter differ (P < 0.05, n = 30)