Fig. 3

Average daily gain. Thirty female BABL/C mice, 5 weeks old, were randomly divided into three groups and immunized subcutaneously with 200 μL of the purified proteins or inactivated virus. Two weeks following fourth immunization, all the mice were infected with 200 μL of PCV2 at 7 log10 TCID₅₀/mL or 100 μL of PBS by intramuscular injection, respectively. All mice were observed three times daily for changes in physical appearance and deaths (if any) for up to 14 days post virus exposure. Generally, mice were weighed every other day to determine the average weight gain of the group