Fig. 1
From: Tween-20 transiently changes the surface morphology of PK-15 cells and improves PCV2 infection
Effect of Tween-20 on PCV2 infection in PK-15 cells. PK-15 cells were treated with or without 0.03% Tween-20 for 24 h, and simultaneously infected PCV2 (MOI = 0.5) for 1 h. After a 24-h treatment, the mixed solution of Tween-20 and PCV2 was washed off and the PK-15 cells were further incubated in cell culture medium containing 2% CS. After 72 h post treatment, the PCV2-infected cells were assessed using an immunofluorescence assay. The number of PCV2-infected cells from PK-15 cells treated with 0.03% Tween-20 was significantly higher compared to PCV2-infected PK-15 cells without Tween-20 treatment (p < 0.01, Table 1). a PCV2-infected PK-15 cells without Tween-20 treatment as control. b PCV2-infected PK-15 cells treated with Tween-20. Magnification: × 100