Table 1 Primer sequences for qPCR
From: Modulation of ovine SBD-1 expression by Saccharomyces cerevisiae in ovine ruminal epithelial cells
Gene names | GenBank accession | Fragment size (bp) | Primer pair sequences (5`-3`) |
|---|---|---|---|
SBD-1 | U75250 | 206 | F: GGCTCCATCACCTGCTCCTC |
R: CGTCTTCGCCTTCTGTTACTTCTT | |||
β-actin | U39357 | 208 | F: GTCACCAACTGGGACGACA |
R: AGGCGTACAGGGACAGCA | |||
TLR2 | DQ890157.1 | 190 | F: GTGTCCGCCGTGTGCTGTGC |
R: AGTAGGAATCCCGCTCGCTGTAGG | |||
MyD88 | GQ221044.1 | 203 | F: AGGTGCCGTCGGATGGTGGTGGTT |
R: TGGTGGCAGGGGTTAGTGTAGTCA | |||
NF-κB | XM_012119628.1 | 95 | F: CACCTTCTCCCAGCCCTTTG |
R: TGCCACCTCCTCCTCCAG | |||
p38 | NM_001142894.1 | 74 | F: CGTTCAGTTCCTTATCTACCAG |
R: GCTCACAGTCTTCATTCACAG | |||
JNK | XM_004002020.3 | 113 | F: ATGACTGCAAAGATGGAAACGA |
R: ATGCTCTGCTTCAGAATCTTGG | |||
ERK1/2 | XM_012157699.1 | 90 | F: GCGCTACACCAATCTCTCGT |
R: ATGGCGACTCGGACTTTGTT |