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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Modulation of ovine SBD-1 expression by Saccharomyces cerevisiae in ovine ruminal epithelial cells

Fig. 2

S.c induces SBD-1 mRNA and protein expression. a Ovine ruminal epithelial cells treated with S.c (5.2 × 108, 5.2 × 107, 5.2 × 106, 5.2 × 105, 5.2 × 104 CFU∙mL− 1) for 2, 4, 8, 12, or 24 h compared to untreated controls. qPCR analysis showed that S.c induces SBD-1 mRNA expression in a time- and concentration-dependent manner. b Ruminal epithelial cells treated with S.c (5.2 × 108, 5.2 × 107, 5.2 × 106, 5.2 × 105, 5.2 × 104 CFU∙mL− 1) for 2, 4, 8, 12, or 24 h compared to untreated controls. ELISA showed that expression of SBD-1 protein was consistent with that observed for SBD-1 mRNA. All experiments were repeated at least 3 times. *P < 0.05, **P < 0.01 vs. the control group

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