Date | Clinical Observation | Intervention | Samples Collected |
---|---|---|---|
Aug 19-20 | Red-crowned crane Aa: emaciation, reduced appetite. Diagnostic check: Heart rate: 106 bp/min Body temperature: 39.7°C Weight: 1.6kg; Crane Aa: astasia. | Rocephin (100 mg/kg) + Dexamethasone (0.25 mg/kg) + 0.9% NaCl 15 ml (intravenous infusion once daily for 3 days) Vitamin C (100 mg/kg) + Vitamin B1(15 mg/kg) + ATP (5 mg/kg) + Inosine (15 mg/kg) + coenzyme A (15 IU/kg) + 10% glucose 15 ml (intravenous infusion once daily for 3 days) 10% glucose + mixture of fish and corn, orally, for 3 days Calcium gluconate (25 mg/kg) + 10% glucose 15 ml (intravenous infusion once daily), for 1 day | Crane Aa: •Histopathological examination of liver, heart, and spleen. Three repeated samples were collected from each tissue. The ratio of MDV-positive tissues: Heart: 67%, liver: 100%, spleen: 67%. •RNA extraction of feather, liver, spleen, kidney and muscle. Three of each. Positive ratio of all samples was 100%. •Genome extraction of muscle, feather, feather-pulp and spleen; three of each. The positive ratio of all samples was 100%. Crane Bb: same as crane A. |
Aug 21 | Diagnostic check: Heart rate: 156 bp/min Body temperature: 39.8°C Crane Aa died. | ||
Aug 22 | Clinical necropsy: Nodules found in skin, muscle, liver, heart, trachea, and gizzard. (MD suspected). | ||
Sept 11 | Crane Bb: obvious swelling of its left knee joint. | Musk analgesic aerosol, external use | |
Sept 12-17 | Clinical improvement: Recovered appetite, left knee joint swelled | Musk analgesic aerosol + ichthammol ointment, external use Calcium tablets (10 mg/kg) + Vitamin D2(500 IU/kg) + Vitamin A (1500 IU/kg), orally | |
Sept 18-19 | Relapse, anorexia, depression | ||
Sept 20 | Crane Bb died. Clinical necropsy: Inflammatory exudates; diffuse yellow-white nodules in muscle, liver, spleen, kidney, pancreas, gizzard, and heart. |