Experimental infection of pigs with H1 and H3 influenza A viruses of swine by using intranasal nebulization

BMC Veterinary Research

Table 3 Serologic analyses of sera obtained 3 days before (Pre) and 14 days post-infection (dpi)

Virus	Pig ID	HAI titer^a		S:N of ELISA^b
Virus	Pig ID	Pre	14 dpi	Pre		14 dpi
Yam10(H1N1)	1	< 10	80	0.95	–	0.72	–
	2	< 10	80	0.86	–	0.88	–
	3	< 10	80	0.97	–	0.68	–
	4	< 10	80	0.88	–	0.85	–
Kag15(H1N1)	1	< 10	20	0.96	–	0.73	–
	2	< 10	160	0.89	–	0.31	+
	3	< 10	160	0.91	–	0.61	–
	4	< 10	80	0.93	–	0.53	+
Toc08(H1N2)	1	< 10	20	1.04	–	0.76	–
	2	< 10	20	1.02	–	0.62	–
	3	< 10	10	0.91	–	0.66	–
	4	< 10	10	1.06	–	0.66	–
Toc12(H1N2)	1	< 10	80	0.85	–	0.71	–
	2	< 10	80	0.78	–	0.56	+
	3	< 10	160	0.96	–	0.39	+
	4	< 10	80	0.90	–	0.52	+
Chi15(H1N1)	1	< 10	160	1.08	–	0.59	+
	2	< 10	80	0.91	–	0.56	+
	3	< 10	160	0.87	–	0.55	+
	4	< 10	80	0.86	–	0.56	+
Nag00(H3N2)	1	< 10	160	0.94	–	0.79	–
	2	< 10	80	0.71	–	0.74	–
	3	< 10	80	0.70	–	0.72	–
	4	< 10	160	0.84	–	0.44	+
Miy13(H3N2)	1	< 10	320	0.72	–	0.78	–
	2	< 10	320	1.02	–	0.78	–
	3	< 10	160	0.70	–	0.59	+
	4	< 10	160	0.73	–	0.72	–
Yok11(H3N2)	1	< 10	320	0.93	–	0.74	–
	2	< 10	80	0.93	–	0.70	–
	3	< 10	160	0.95	–	0.62	–
	4	< 10	160	0.82	–	0.47	+
Min06(H3N2)	1	< 10	320	1.00	–	0.72	–
	2	< 10	320	0.68	–	0.96	–
	3	< 10	160	0.93	–	0.96	–
	4	< 10	80	0.75	–	0.49	+
Control	1	< 10	< 10	0.84	–	0.95	–
	2	< 10	< 10	0.82	–	0.93	–
	3	< 10	< 10	0.82	–	0.95	–
	4	< 10	< 10	0.86	–	0.95	–

^aHomologous viruses were used as antigens in each group. Sera from control pigs were tested against classical H1 (Chi15), A(H1N1)pdm09v (Kag15), and triple-reassortant (Yok11) IAVs-S as antigens
^bFor swine sera, a sample:negative (S:N) ratio < 0.6 was considered positive (+; −, negative), according to the manufacturer’s instructions

ISSN: 1746-6148