In vitro inhibition of porcine reproductive and respiratory syndrome virus replication by short antisense oligonucleotides with locked nucleic acid modification

Table 1 List of oligonucleotides used in this study

Name of oligonucleotides	Sequence (from 5′ to 3′)	Target gene	Position within the target gene	GC content (%)	Tm (°c)
YN8	TGCAGCATCCTCACAACCGT	Nsp9	704–723 bp	55	65.3
LNA-YN8-A	TGCAGCATCCTCACAACCGT				71.8
LNA-YN8-B	TGCAGCATCCTCACAACCGT				71.2
ACTB-F	agttgcgttacaccctttcttga	beta-actin	1168–1190	43.5	58
ACTB-R	tgctgtcaccttcaccgttc	beta-actin	1297–1316	55	59.4
ORF7-F	aatggccagccagtcaatca	ORF7	14,844–14,863	50	58.5
ORF7-R	tcacgctgagggcgatgctg	ORF7	15,154–15,173	65	65

ISSN: 1746-6148