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Fig. 5 | BMC Veterinary Research

Fig. 5

From: In vitro inhibition of porcine reproductive and respiratory syndrome virus replication by short antisense oligonucleotides with locked nucleic acid modification

Fig. 5

Indirect immunofluorescence detection of PRRSV in Marc-145 cells transfected with antisense oligonucleotides YN8 (a), LNA-YN8-A (b) or LNA-YN8-B (c). Cells with neither infection nor transfection and cells with infection but without transfection (d) serve as controls. Transfection was performed 90 min post infection with PRRSV YN-1 strain (25 TCID50). Cells were fixed 60 h post transfection. Anti-N monoclonal antibody (mAb) and Alexa Fluo488 conjugated secondary antibody were applied in the indirect immunofluorescence staining. Blue color stands for the nuclei and the green color indicates the expression of N protein in Marc-145 cells. The images of the same treatment from the two channels were merged. Treatment with LNA-YN8-A or LNA-YN8-B resulted in further reduction of virus replication, compared with the antisense oligonucleotide YN8 without LNA modifications. These images are representative for 3 independent experiments. Quantification data with statistical analysis of a, b and c was shown in e

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BMC Veterinary Research

ISSN: 1746-6148

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