Skip to main content

Advertisement

Fig. 1 | BMC Veterinary Research

Fig. 1

From: Identification of immunologic and clinical characteristics that predict inflammatory response to C. Novyi-NT bacteriolytic immunotherapy

Fig. 1

Example gating scheme for identification of CD3+/CD4+ lymphocytes and CD21+ lymphocytes. Lymphocytes were identified and gated using a forward and side scatter plot (a). Gated lymphocytes were applied were then applied to PE (CD3) vs FITC (CD4) plots or an Alexa Fluor 647 (CD21) histogram, respectively. Unstained cells and matched isotype controls from the same manufacturer were used to determine cut offs for negative (b and d) versus positive (c and e) cells. CD3+/CD4+ or CD21 positive cells were then identified. CD3+/CD8+ cells were selected in the same manner as the CD3+/CD4+ cells

Back to article page