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Table 1 Technical data and references of molecular methods used in this study

From: Tick- and fly-borne bacteria in ungulates: the prevalence of Anaplasma phagocytophilum, haemoplasmas and rickettsiae in water buffalo and deer species in Central Europe, Hungary

Target pathogen categories (target gene) Oligonucleotides (sequence 5′-3′) Reference for original method (modified protocol)
Anaplasma phagocytophilum
(msp2)
ApMSP2f (ATG GAA GGT AGT GTT GGT TAT GGT ATT)
ApMSP2r (TTG GTC TTG AAG CGC TCG TA)
ApMSP2p (TGG TGC CAG GGT TGA GCT TGA GAT TG-HEX)
([13]) [35]
haemoplasmas
(16S rRNA)
Sybr_For (AGC AAT RCC ATG TGA ACG ATG AA)
Sybr_Rev1 (TGG CAC ATA GTT TGC TGT CAC TT)
Sybr_Rev2 (GCT GGC ACA TAG TTA GCT GTC ACT)
[36] ([37])
Mycoplasma wenyonii, M. ovis
(16S rRNA)
MwenyoniiF (CCA CGT GAA CGA TGA AGG TCT T)
MwenyoniiR (GGC ACA TAG TTA GCT GTC ACT TAT TCA A)
Mweny_P (FAM-AGT ACC ATC AAG GCG CGC TCA TTT CCT AG-TAMRA)
[38]
Candidatus M. haemobos’
(16S rRNA)
Mwen_short.forw (CCA TGT GAA CGA TGA AGG TCT TT)
Mwen_short.rev (AGT TTG CTG TCA CTT ATT CAT GAG GTA)
Mwen_short.p (VIC-CTA TCA GTT RTT ATC CCT CAT AA-MGB)
[38]
Mycoplasma suis
(16S rRNA)
RTsuisF (CCC TGA TTG TAC TAA TTG AAT AAG)
RTsuisR (GCG AAC ACT TGT TAA GCA AG)
MGBsuis2 (FAM- TGR ATA CAC AYT TCA G -MGBNFQ)
[18]
Rickettsia helvetica
(23S rRNA)
Rickhelv.147f (TTT GAA GGA GAC ACG GAA CAC A)
Rickhelv.211r (TCC GGT ACT CAA ATC CTC ACG TA)
Rickhelv.170p (6FAM-AAC CGT AGC GTA CAC TTA-MGBNFQ)
[39]
other rickettsiae
(gltA)
CS-F (TCG CAA ATG TTC ACG GTA CTT T)
CS-R (TCG TGC ATT TCT TTC CAT TGT G)
CS-P (FAM-TGC AAT AGC AAG AAC CGT AGG CTG GAT G-BHQ)
[39]