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Fig. 5 | BMC Veterinary Research

Fig. 5

From: Pathologic and biochemical characterization of PrPSc from elk with PRNP polymorphisms at codon 132 after experimental infection with the chronic wasting disease agent

Fig. 5

RT-QuIC detection of seeding activity in elk brain samples using mature length recombinant elk prion protein 132 L (a) and 132 M (b) substrates. RT-QuIC assays seeded with MM132 (green), LM132 (red), LL132 (black) or negative seed (yellow) are shown. RT-QuIC reaction mixtures were seeded with 10− 2 dilutions of normalized brain homogenate. A final 400 mM NaCl was used with each substrate. Data are presented as mean ThT fluorescence of quadruplicate reactions

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BMC Veterinary Research

ISSN: 1746-6148

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