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Table 1 Adaptation of H9N2 avian influenza virus in SPF embryonated chicken eggs with or without SPA

From: Evolution of H9N2 avian influenza virus in embryonated chicken eggs with or without homologous vaccine antibodies

Characters

F/98

nF47

nF52

vF47

HI titer

512

256

16

16

Change in HI titer compare to F/98

 

2-fold

32-fold

32-fold

EMDT (h)a

86

79

68.5

75

Change in EMDT(h)compare to F/98

 

7

17.5

11

EID50 (log10 EID50/0.2 ml)

6.67

7.64

8.74

7.77

Changed in EID50 compare to F/98 (log10 decrease)

 

9.3-fold**

117.5-fold**##

12.5 fold**

Mutationsb compare to F/98 (start-end passage of mutant)

 

PB2: S155 N, C228Y, K375R (nF46-nF80)

PB2:S155 N, C228Y, K375R (nF46-nF80)

PB2: I185K, C228Y, K375R (vF43-vF80)

  

PB1: none

PB1:T156A, R189K (nF49-nF80)

PB1: none

  

PA: none

PA: none

PA: none

  

HA: K131R (nF30)

HA: K131R(nF30-nF80), S145 N, G181E, A198V (nF52-nF80)

HA:

K131R, S145 N, G181E, A198V (vF47-vF80)

  

NP: none

NP: R99K (nF50-nF80)

NP: none

  

NA: none

NA: A459T (nF52-nF80)

NA: none

  

M: none

M: none

M: none

  

NS1: none

NS1: A143T (nF50-nF80)

NS1: none

  1. aEMDT: Embryo mean death time
  2. beach gene represent results from 10 clones
  3. **P < 0.01 for the 50% egg infectious dose of nF47, vF47, and nF52 was significantly higher than that of their parental virus F/98
  4. ##P < 0.01 for the 50% egg infectious dose of nF52 was significantly higher than that of nF47 and vF47