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Table 1 Primary antibodies used for immunofluorescence and western blotting

From: Characterisation of feline renal cortical fibroblast cultures and their transcriptional response to transforming growth factor β1

Antibody Supplier Secondary antibody Dilution for IF Dilution for western blot Incubation temperature for western blot
Cytokeratin AE1/AE3 Dako Mouse 1:100 1:1000 4 °C
Vimentin
(V9)
Dako Mouse 1:100 1:1000 4 °C
Desmin
(DE-R-11)
Dako Mouse 1:100 N/A N/A
CD29
(TS2/16)
Bio-rad Mouse 1:100 1:500 4 °C
CD44
(156-3C11)
ABD serotec Rat 1:100 N/A N/A
vWF Dako Rabbit 1:200 1:1000 4 °C
VCAM-1 R&D Mouse 1:100 N/A N/A
Rabbit Polyclonal Isotype control Biolegend Rabbit 1:50–1:200 N/A N/A
Mouse IgG1 κ isotype control Biolegend Mouse 1:50–1:200 N/A N/A
β-actin
(AC-74)
Sigma-Aldrich Mouse N/A 1:5000 4 °C