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Table 4 Nucleotide mutations and corresponding amino acid substitutions of Staphylococcus aureus from cases of clinical mastitis

From: Virulence gene profiles: alpha-hemolysin and clonal diversity in Staphylococcus aureus isolates from bovine clinical mastitis in China

Geno type

No.

Nucleotide mutation position/corresponding amino acid substitutiona

−42

−22

−6 &-5

47

144

214

237

255

262

399

438

453

498

499

519

606

686

765

797

824

833

C

G

GG

G

C

G

C

G

T

C

G

T

C

T

T

T

C

T

A

C

T

1

65

−b

A

AA

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

   

Sb

G(−2)N

                  

2

5

–

A

AA

–

–

–

–

–

C

T

C

G

–

–

–

C

–

C

–

–

–

   

S

G(−2)N

     

S

S

S

S

   

S

 

S

   

3

4

T

A

AA

–

–

–

–

–

–

T

C

G

–

–

–

C

–

C

–

–

–

  

S

S

G(− 2)N

      

S

S

S

   

S

 

S

   

4

4

–

A

AA

–

–

–

–

–

–

–

–

–

T

–

–

–

–

–

–

–

–

   

S

G(−2)N

         

S

        

5

3

–

A

AA

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

A

   

S

G(−2)N

               

S278Y

6

2

–

A

AA

–

–

–

–

A

–

–

–

–

–

–

–

–

–

–

–

–

–

   

S

G(−2)N

    

S

             

7

2

–

A

AA

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

T

   

S

G(−2)N

               

S278F

8

2

–

A

AA

–

T

–

–

–

–

–

–

–

–

–

–

C

–

C

C

T

–

   

S

G(−2)N

 

S

          

S

 

S S T275I

9

1

–

A

AA

–

–

A

–

A

–

–

–

–

–

–

–

–

–

–

–

–

–

   

S

G(−2)N

 

Q72S

S

             

10

1

–

A

AA

T

–

–

–

–

–

T

C

G

–

–

–

C

–

C

–

–

–

   

S

G(−2)N

S16I

   

S

S

S

   

S

 

S

   

11

1

–

A

AA

–

–

–

A

–

–

–

–

–

–

–

–

–

–

–

–

–

–

   

S

G(−2)N

   

S

              

12

1

–

A

AA

–

–

–

–

–

–

–

–

–

–

–

–

–

G

–

–

–

A

   

S

G(−2)N

            

A229G

S278Y

13

1

–

A

AA

–

–

–

–

–

–

–

–

–

–

–

A

–

–

–

–

–

–

   

S

G(−2)N

          

N173 K

     

14

1

–

A

AA

–

–

–

–

–

–

–

–

–

–

Db

–

–

–

–

–

–

–

   

S

G(−2)N

         

W167Gc

     
  1. aNucleotide/peptide positions were designated relative to the first nucleotide/amino acid of the mature 훂-hemolysin (S. aureus WOOD46)
  2. bS synonymous mutation, D deletion mutation, − = no mutation;
  3. cThe deletion at nucleotide position 499 of Genotype 14 resulted in peptide termination at residue position 169