Virulence gene profiles: alpha-hemolysin and clonal diversity in Staphylococcus aureus isolates from bovine clinical mastitis in China

Table 4 Nucleotide mutations and corresponding amino acid substitutions of Staphylococcus aureus from cases of clinical mastitis

Geno type	No.	Nucleotide mutation position/corresponding amino acid substitution^a
		−42	−22	−6 &-5	47	144	214	237	255	262	399	438	453	498	499	519	606	686	765	797	824	833
		C	G	GG	G	C	G	C	G	T	C	G	T	C	T	T	T	C	T	A	C	T
1	65	−^b	A	AA	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–
			S^b	G(−2)N
2	5	–	A	AA	–	–	–	–	–	C	T	C	G	–	–	–	C	–	C	–	–	–
			S	G(−2)N						S	S	S	S				S		S
3	4	T	A	AA	–	–	–	–	–	–	T	C	G	–	–	–	C	–	C	–	–	–
		S	S	G(− 2)N							S	S	S				S		S
4	4	–	A	AA	–	–	–	–	–	–	–	–	–	T	–	–	–	–	–	–	–	–
			S	G(−2)N										S
5	3	–	A	AA	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	A
			S	G(−2)N																S278Y
6	2	–	A	AA	–	–	–	–	A	–	–	–	–	–	–	–	–	–	–	–	–	–
			S	G(−2)N					S
7	2	–	A	AA	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	–	T
			S	G(−2)N																S278F
8	2	–	A	AA	–	T	–	–	–	–	–	–	–	–	–	–	C	–	C	C	T	–
			S	G(−2)N		S											S		S S T275I
9	1	–	A	AA	–	–	A	–	A	–	–	–	–	–	–	–	–	–	–	–	–	–
			S	G(−2)N		Q72S			S
10	1	–	A	AA	T	–	–	–	–	–	T	C	G	–	–	–	C	–	C	–	–	–
			S	G(−2)N	S16I						S	S	S				S		S
11	1	–	A	AA	–	–	–	A	–	–	–	–	–	–	–	–	–	–	–	–	–	–
			S	G(−2)N				S
12	1	–	A	AA	–	–	–	–	–	–	–	–	–	–	–	–	–	G	–	–	–	A
			S	G(−2)N													A229G			S278Y
13	1	–	A	AA	–	–	–	–	–	–	–	–	–	–	–	A	–	–	–	–	–	–
			S	G(−2)N											N173 K
14	1	–	A	AA	–	–	–	–	–	–	–	–	–	–	D^b	–	–	–	–	–	–	–
			S	G(−2)N										W167G^c

^aNucleotide/peptide positions were designated relative to the first nucleotide/amino acid of the mature 훂-hemolysin (S. aureus WOOD46)
^bS synonymous mutation, D deletion mutation, − = no mutation;
^cThe deletion at nucleotide position 499 of Genotype 14 resulted in peptide termination at residue position 169

ISSN: 1746-6148