Fig. 4

Cellular cholesterol and PRV adsorption. MA104 cells (PRV) and BHK-21 cells (VSV) were either mock treated or treated with 8 mM or 15 mM MβCD for 30 min, then infected with PRV or VSV (100 TCID₅₀) at 4 °C for 2 h. Antibodies to the PRV VP7 or VP4 proteins and to the VSV G protein were used to screen western blots of cell homogenates and virus titers. a Virus titers of PRV and VSV after treatment. b Western blot of PRV VP7 and VP4 proteins and β-actin. c Western blot of VSV G protein and β-actin. Western blots depict representative experiments. Error bars (Fig. 4a) indicate the standard deviations of three independent experiments