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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Cholesterol of lipid rafts is a key determinant for entry and post-entry control of porcine rotavirus infection

Fig. 2

The restoration of PRV-infectivity by exogenous cholesterol. MA104 cell and BHK-21 cell monolayers were treated with 15 mM MβCD before supplemented with exogenous cholesterol. Subsequently, cells were infected with PRV or VSV (100 TCID50) respectively, except mock as control without virus infection and MβCD/ exogenous cholesterol treatment. a IFA data demonstrating infectivity of PRV and VSV using anti-VP7 antibodies and anti-VSV G protein antibodies respectively. The 100% infectivity values of PRV and VSV represent average fluorescein-stained cells numbers of 160 or 230, respectively; b Real-time PCR data for PRV and VSV targeting the VP7 and G protein gene. Error bars indicate the standard deviations of three independent experiments

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