Real-time fluorescence loop-mediated isothermal amplification assay for direct detection of egg drop syndrome virus

Table 1 RealAmp and PCR primers

Method	Primer name	Length (bp)	Sequence (5′-3′)	Location of the primers^a
RealAmp	F3	20	AAAGGTTGCAGGGTATGTGT	24,070–24,089
	B3	18	TAATGGCATTGGCCGCAA	24,297–24,314
	FIP (F2)	20	GTTGGTGGGCTTGTACATGG	24,101–24,120
	FIP (F1c)	22	TTCCCCCCGTAAACCAATACCC	24,146–24,167
	BIP (B2)	20	TCACCACTCCACACTACTGG	24,257–24,276
	BIP (B1c)	22	TGTCCTTTTAGTGCTCGCGACC	24,206–24,227
	LF	25	CGCAGTAGCTTTAATCTGAATGGTC	24,121–24,145
	LB	20	CCACTGCTAACCTGTCAGGC	24,228–24,247
PCR	Fiber-F	20	ATGAAGCGACTACGGTTGGA	22,685–22,704
PCR	Fiber-R	26	CTACTGTGCTCCAACATATGTAAAGG	24,594–24,619

F3- forward outer primer, B3- backward outer primer, LF- loop forward primer, LB- loop backward primer, FIP- forward inner primer and BIP- backward inner primer. F denotes forward primer and R denotes reverse primer, ^alocations of primers in EDSV genome

ISSN: 1746-6148