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Fig. 4 | BMC Veterinary Research

Fig. 4

From: Real-time fluorescence loop-mediated isothermal amplification assay for direct detection of egg drop syndrome virus

Fig. 4

Specificity test of the real-time fluorescence loop mediated isothermal amplification assay for the detection of EDSV. a specificity of RealAmp among different virus strains; tube 1 negative control (ddH2O); tube 2 FPV; tube 3 DVEV; tube 4 DCV; tube 5 MDV; tube 6 EDSV and tube 7 positive control (EDSV DNA). b 1.5% agarose gel electrophoresis of RealAmp products; lane 1 negative control; lane 2 FPV; lane 3 DVEV; lane 4 DCV; lane 5 MDV; lane 6 EDSV and lane 7 positive control. c Validation of RealAmp specificity. Lane 1 digested RealAmp product; lane 2 undigested RealAmp product and lane M Trans 2 K plus DNA marker

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BMC Veterinary Research

ISSN: 1746-6148

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