Fig. 3
Direct RealAmp assay for allantoic fluid and cell culture supernatant. a Infected allantoic fluid; (b) cell culture supernatant with serial 10-fold dilutions, tube 1–5 were sample dilutions (10− 1 to 10− 5); tube 6 undiluted sample; tube 7 positive (EDSV DNA) and tube 8 (uninfected) control. c and (d) 1.5% Agarose gel electrophoresis results of (a) and (b), lanes 1–5, sample dilutions (10− 1 to 10−5); lane 6 undiluted samples; lane 7 positive and lane 8 negative controls. M- Trans2K plus DNA marker