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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Real-time fluorescence loop-mediated isothermal amplification assay for direct detection of egg drop syndrome virus

Fig. 2

RealAmp using viral DNA isolated from infected allantoic fluid and cell suspension. a RealAmp of viral DNA extracted from allantoic fluid; (b) RealAmp of viral DNA from infected duck fibroblast cell culture supernatant used with serial dilutions. For both reactions, tube 1–6 DNA sample dilutions (10− 1 to 10− 6); tube 7 for positive (EDSV DNA) and tube 8 negative (uninfected) controls. c and (d) 1.5% agarose gel electrophoresis results of (a) and (b), lane 1–6, sample dilutions (10− 1 to 10− 6); lane 7 positive control; lane 8 negative control; lane M- Trans2K plus DNA marker

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