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Fig. 3 | BMC Veterinary Research

Fig. 3

From: Cortisol modulates inflammatory responses in LPS-stimulated RAW264.7 cells via the NF-κB and MAPK pathways

Fig. 3

Effects of cortisol on the mRNA and protein expression levels of iNOS and COX-2 in LPS-stimulated RAW264.7 cells. a and b Cells were co-treated with cortisol (5,15 and 30 ng/mL) and LPS (1 μg/mL) for 0, 6, 12, and 24 h. RNA was isolated and analyzed by RT-PCR. c and d Cells were co-treated with cortisol (5, 15 and 30 ng/mL) and LPS (1 μg/mL) for 24 h. Total proteins were isolated and analyzed by Western blot. The data presented are the means±SEM. ** p < 0.01 vs. the control group; # p < 0.05, ## p < 0.01 vs. the LPS group

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