Skip to main content

Table 3 Mean quantification cycle (Cq) values resulting from MGB PCRa

From: A rapid minor groove binder PCR method for distinguishing the vaccine strain Brucella abortus 104M

Detection of 104M genomic DNA Detection of A19 genomic DNA
Concentration Cq of FAM Cq of VIC Concentration Cq of FAM Cq of VIC
1.1 ng/μL 24.06 NA 3.8 ng/μL NA 17.49
110 pg/μL 27.20 NA 380 pg/μL NA 20.89
11 pg/μL 30.92 NA 38 pg/μL NA 24.39
1.1 pg/μL 35.27 NA 3.8 pg/μL NA 31.90
110 fg/μL 38.25 NA 380 fg/μL NA 34.69
11 fg/μL NA NA 38 fg/μL NA 38.11
1.1 fg/μL NA NA 3.8 fg/μL NA NA
0.11 fg/μL NA NA 0.38 fg/μL NA NA
  1. aFAM = 6-carboxyfluorescein; VIC = 4,7,2′-trichloro-7′-phenyl-6-carboxyfluorescein; NA = not applicable