Fig. 1From: A rapid minor groove binder PCR method for distinguishing the vaccine strain Brucella abortus 104MSpecificity of the MGB PCR assay using representative experiments for the detection of (a) FAM fluorescence and (b) VIC fluorescence. The following strains were subjected to amplification assays: Line 1, 104 M; Line 2 Brucella suis biovar 1 S1330; Line 3, B. suis biovar 2 Thomsen; Line 4, B. suis biovar 3686; Line 5, B. suis biovar 4 40; Line 6, B. abortus biovar 1 A544; Line 7, B. abortus biovar 2 86/8/59; Line 8, B. abortus biovar 3 Tulya; Line 9, B. abortus biovar 4292; Line 10, B. abortus biovar 5 B3196; Line 11, B. abortus biovar 6870; Line 12, B. abortus biovar 7 63/75; Line 13, B. abortus biovar 9 C68; Line 14, B. melitensis biovar 1 16 M; Line 15, B. melitensis biovar 2 63/9; Line 16, B. melitensis biovar 3442; Line 17, B. ovis 63/290; Line 18, B. canis RM6/66; Line 19, B. neotomae 5 K33; Line 20, S2; Line 21, S19; Line 22, A19; Line 23, M5; Line 24, S2; Line 25, Escherichia coli K99; Line 26, Pasteurella multocida C48–1; Line 27, Streptococcus suis ST171; Line 28, Pseudomonas aeruginosa DI-1Back to article page