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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera

Fig. 1

Morphology and stemness features of OE-MSCs from different mammalian genera. After 4 weeks in growth culture medium, olfactory mucosa explants formed homogeneous populations of adherent and highly proliferative cells exhibiting a mesenchymal-like fibroblastic morphology: examples of sheep (a). When grown under appropriate culture conditions, OE-MSCs could generate spheres: examples of rabbit (b). After seven passages, cells express the nestin protein (in green, (c) example of rabbit), a prominent marker of immaturity. OE-MSCs were immunostained with 3 surface markers, quantified using a flow cytometer and expression level compared to isotype: example of macaque (d). Each image is representative of multiple independent cultures of each species. Scale bar: 200 μm (a & b), 100 μm (c)

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