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Table 1 Ratio of MiCV-positive serum to MiCV-negative serum for the optimization of assay conditions for the ELISA against recombinant rCap antigen

From: Development and application of an indirect enzyme-linked immunosorbent assay based on recombinant capsid protein for the detection of mink circovirus infection

Serum dilution Concentration of rCap antigen (μg/well)
4 2 1 0.5 0.25 0.125
1:25(+) 0.806 1.056 1.048 0.969 0.89 0.742
1:25(−) 0.108 0.166 0.16 0.153 0.133 0.104
1:50(+) 0.876 1.175 1.044 0.967 0.888 0.78
1:50(−) 0.175 0.116 0.144 0.12 0.116 0.09
1:100(+) 0.753 1.078 1.063 0.964 0.895 0.737
1:100(−) 0.096 0.121 0.129 0.1 0.114 0.089
1:200(+) 0.971 1.119 1.065 1.004 0.801 0.767
1:200(−) 0.113 0.127 0.15 0.108 0.097 0.088
1:400(+) 0.681 0.724 0.807 0.779 0.821 0.638
1:400(−) 0.094 0.068 0.111 0.083 0.083 0.068
  1. Optimum values with the best positive-to-negative ratio are highlighted