|
Serum dilution
|
Concentration of rCap antigen (μg/well)
|
|---|
|
4
|
2
|
1
|
0.5
|
0.25
|
0.125
|
|---|
|
1:25(+)
|
0.806
|
1.056
|
1.048
|
0.969
|
0.89
|
0.742
|
|
1:25(−)
|
0.108
|
0.166
|
0.16
|
0.153
|
0.133
|
0.104
|
|
1:50(+)
|
0.876
|
1.175
|
1.044
|
0.967
|
0.888
|
0.78
|
|
1:50(−)
|
0.175
|
0.116
|
0.144
|
0.12
|
0.116
|
0.09
|
|
1:100(+)
|
0.753
|
1.078
|
1.063
|
0.964
|
0.895
|
0.737
|
|
1:100(−)
|
0.096
|
0.121
|
0.129
|
0.1
|
0.114
|
0.089
|
|
1:200(+)
|
0.971
|
1.119
|
1.065
|
1.004
|
0.801
|
0.767
|
|
1:200(−)
|
0.113
|
0.127
|
0.15
|
0.108
|
0.097
|
0.088
|
|
1:400(+)
|
0.681
|
0.724
|
0.807
|
0.779
|
0.821
|
0.638
|
|
1:400(−)
|
0.094
|
0.068
|
0.111
|
0.083
|
0.083
|
0.068
|
- Optimum values with the best positive-to-negative ratio are highlighted
