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Table 1 Primer sequences, amplicon length (AE), annealing temperature (T), efficiency (E) and gene reference

From: Effects of the isoflavone genistein in early life stages of the Senegalese sole, Solea senegalensis: role of the Survivin and proliferation versus apoptosis pathways

Target

Sequence (5’→3′)

AE (bp)

T (°C)

Ea

Gene reference

BIRC-5

TGATCCAGAAAAGGAGCACA (F);

TGGTGTCGTTGCAGGATTTA (R)

149

56

2.0799

solea_v4.1_unigene59754*

Caspase-6

CAGGACATCACAGCCATGTT (F);

TCACTGTCCACAGCATCACA (R)

134

60

2.0205

solea_v4.1_unigene3643*

Fas

AGCCACTGCTCTGTGAAACC (F);

CTAGGTTTGCGTTGGGATGT (R)

130

60

2.0403

solea_v4.1_unigene23355*

  1. a100% efficiency is E = 2 and corresponds to a slope of −3.32. to accept the standar curve the R2 value must be >0.99
  2. *Database Solea BD. http://www.juntadeandalucia.es/agriculturaypesca/ifapa/soleadb_ifapa/
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BMC Veterinary Research

ISSN: 1746-6148

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