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Fig. 4 | BMC Veterinary Research

Fig. 4

From: Acute-onset high-morbidity primary photosensitisation in sheep associated with consumption of the Casbah and Mauro cultivars of the pasture legume Biserrula

Fig. 4

Photomicrographs of the alopecic and oedematous areas of the ear pinnae: (a) Low magnification showing severe haemorrhage and oedema in the dermis. H&E, objective × 1.25. b Micropustules containing neutrophils, eosinophils and necrotic debris in the stratum corneum and upper stratum granulosum on the external aspect of the ear pinna. Mild individual cell keratinocyte necrosis, mild acanthosis and haemorrhage in the upper dermis are also shown. H&E, × 200. c Moderate zonal necrosis of the outer layers of the epidermis, which is infiltrated by numerous eosinophils and neutrophils, at places forming small intraepidermal pustules. Keratinocytes show moderate individual cell necrosis. H&E, × 400. (d) Severe epidermal necrosis with obliteration of the follicular epithelial structure and sebaceous gland necrosis (arrows) in the most severely affected section of the ear pinna. Intraepidermal nodular pustules formed by dead and viable neutrophils; eosinophils can be seen multifocally. The dermis is infiltrated by eosinophils and neutrophils, particularly adjacent to necrotic hair follicles. H&E, × 200

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