Fig. 3From: Biopanning of polypeptides binding to bovine ephemeral fever virus G1 protein from phage display peptide libraryInhibitory effects of selected phage on BEFV detected by real-time RT-PCR. 11 selected phages labeled Phage T25,T20,T19,T26,T24,T17,T18,T15,T21,T27 and T8 were incubated with 100TCID50/0.1 mL, respectively. The SYBR Green-based quantitative real-time PCR was used to determine BEFV RNA with specific primers for N gene. The relative expression of BEFV was normalized to GAPDH expression and analysed by the 2(−△△Ct) method. Each treated sample was run in triplicate. Statistical analysis was performed by one-way analysis of variance and values were considered significant when p < 0.05. Figures were performed using the GraphPad™ Prism 5.0 software. Statistical significance (p < 0.05) was noted by “*” compared to control groupsBack to article page