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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Biopanning of polypeptides binding to bovine ephemeral fever virus G1 protein from phage display peptide library

Fig. 1

SDS-PAGE and Western blot analysis of purified recombinant G1 protein. Large amounts of highly expressed recombinant G1 protein were purified using Ni2+-sepharose 6 Fast Flow affinity media under native conditions. a SDS-PAGE analyses of purified recombinant G1 protein and pET32a vector protein. M, molecular protein marker; Lane 1, purified recombinant G1 protein; Lane 2, pET32a vector protein. b Western blot analysis of purified recombinant G1 protein and pET32a vector protein react with BEFV antibody. The left lane: vector protein control; the right lane: purified recombinant G1 protein

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